This paper aimed to identify the organizational entrepreneurship barriers of the Gymnastics Federation of the Islamic Republic of Iran. The mixed method and Brown and Clark method were used in the research. The population consisted of two sections, including gymnastics experts and university professors (180 people), and the sample size was 16 people based on the theoretical saturation index and 120 people based on the Cochran formula. Three structural 14, behavioral 13, and contextual barriers with 5 indicators were identified by qualitative data analysis. The research instrument in the quantitative part was a questionnaire that was extracted from the codes of the qualitative part using LISREL and SPSS software. The results confirmed the items and factors in the first and second confirmatory factor analysis tests. Three barriers were identified, and 32 indicators were obtained as the measurement tool. The model was fitted using the structural equation method (SEM) based on path coefficient and t-statistic. The effect of three independent variables was significant (1.00, 0.90, and 0.97), indicating the model’s high estimation power. The results showed that underlying barriers had the most significant effect on the failure of organizational entrepreneurship.